BBa_K210009

BBa_K210009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K210009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Makoto Kashima

Date created: 2009-10-14 11:00:00

Date modified: 2015-05-08 01:11:29

HIV-TAT::(LALAAAA)3 generator

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K210009_sequence (Version 1)

Description

T7promoter(BBa_I712074)+HIV-TAT+his tag+(LALAAAA)??3+terminator(BBa_B0015)

(LALAAAA)??3 is a transmenbrene domein. The liposome which has HIV-TAT can intrude in mammalian cells.
We translated this parts by RTS with liposome, and ultracentrifuge in order to separate the liposome with HIV-TAT.

Notes

This part is identified by his tag.

Source

BBa_I712074,BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7(BBa_I712074)	1,47	feature/promoter, promoter
BBa_B0034	55,66	feature/rbs, ribosome_entry_site
HIV-TAT	73,108	CDS, feature/protein
his tag	109,144	tag, feature/tag
(LALAAAA)??3	145,210	feature/protein, CDS
BBa_B0034	219,347	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K210009/1

Member of these Collections

• iGEM Parts Registry