BBa_K2109108

BBa_K2109108 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2109108

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andy Hudson

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-28 01:07:17

MS2 6XHis (V29I/d1FG)

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K2109108_sequence (Version 1)

Description

this part is an improvement upon BBA_K1791000 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.

Notes

n/a

Source

n/a

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2109108/1

Member of these Collections

• iGEM Parts Registry