BBa_K2109108

BBa_K2109108 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2109108
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andy Hudson
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-28 01:07:17

MS2 6XHis (V29I/d1FG)



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K2109108_sequence (Version 1)

    Description

    this part is an improvement upon BBA_K1791000 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.

    Notes

    n/a

    Source

    n/a

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2109108/1
     

    Attachments

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