| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K2110800_sequence (Version 1)
|
Description
The sequence codes the wild type protein called PETase. PETase is a Poly(ethylene terephthalate) hydrolase, which was found in Ideonella sakaiensis (strain 201-F6) by Japanese scientists. It allows Ideonella sakaiensis to degrade PET. If properly transferred into engineered organisms as E.Coli or yeast, secreted PETase can catalyze the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Optimum temperature is 40 degrees Celsius for PET film hydrolysis and optimum pH is 9.
Notes
This enzyme should be work in extracellular environments.
Source
Come from the genome of Ideonella sakaiensis (strain 201-F6)
