Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fanglin Zhao
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 08:05:06
Fragment 1
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K2112011_sequence (Version 1) |
Description
T7 promoter is a strong promoter to launch the transcription procedure.Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated.
His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily.