BBa_K2114001

BBa_K2114001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-19 02:50:21

aGFPnano_HA_aHelix_cotZ



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2114001_sequence (Version 1)

    Description

    N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ.

    Notes

    Introduction of HA epitope tag and alpha helical linker by primer extensions.

    Source

    Amplification of cotZ from genomic DNA of Bacillus subtilis. The anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS)

    Sequence Annotation Location Component / Role(s)
    anti-GFP nanobody
    HA
    alpha helical linker
    cotZ
    		1,354
    355,381
    382,414
    415,861
    		feature/cds CDS
    tag feature/tag
    feature/misc sequence_feature
    CDS feature/cds

    igem#experience
    Works
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114001/1
     

    Attachments

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