BBa_K2114002

BBa_K2114002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-19 03:31:55

aGFPnano_HA_G4S_cotZ



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2114002_sequence (Version 1)

    Description

    N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ by a flexible GGGGS linker.

    Notes

    Introduction of a HA epitope tag and a flexible GGGGS linker by primer extensions.

    Source

    The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived camelid heavy-chain antibodies.

    Sequence Annotation Location Component / Role(s)
    anti-GFP nanobody
    HA
    GGGGS linker
    cotZ
    		1,354
    355,381
    382,396
    397,843
    		feature/cds CDS
    tag feature/tag
    sequence_feature feature/misc
    CDS feature/cds

    igem#experience
    Works
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114002/1
     

    Attachments

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