BBa_K2114009

BBa_K2114009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-19 09:03:30

aGFPnano_HA_aHelix_cotG



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2114009_sequence (Version 1)

    Description

    N-terminal fusion of anti-GFP nanobody to spore crust gene cotG by a alpha helical linker.

    Notes

    Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.

    Source

    The cotG gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies.

    Sequence Annotation Location Component / Role(s)
    anti-GFP nanobody
    HA
    alpha helical linker
    cotG
    		1,354
    355,381
    382,414
    415,1002
    		feature/cds CDS
    tag feature/tag
    feature/misc sequence_feature
    feature/cds CDS

    igem#experience
    Works
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114009/1
     

    Attachments

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