BBa_K2114013

BBa_K2114013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-12 04:11:49

GST_HA_G4S_CotB



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2114013_sequence (Version 1)

    Description

    N-terminal fusion of glutathione S-transferase to spore crust gene cotB by a flexible GGGGS linker.

    Notes

    Introduction of HA epitope tag and GGGGS linker by primer extensions and PCR.

    Source

    The cotB gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

    Sequence Annotation Location Component / Role(s)
    glutathione S-transferase
    HA
    GGGGS linker
    cotB
    		1,699
    700,726
    727,741
    742,1884
    		feature/cds CDS
    tag feature/tag
    feature/misc sequence_feature
    feature/cds CDS

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K2114013/1
     

    Attachments

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