BBa_K2114014

BBa_K2114014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-09 07:10:32

aGFPnano_HA_G4S_CotB

Details

• Types: DnaRegion
• Roles: CDS; Coding

Description

N-terminal fusion of anti-GFP nanobody to spore crust gene cotB by a flexible GGGGS linker.

Notes

Introduction of HA epitope tag and GGGGS linker by primer extensions and PCR.

Source

The cotB gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 19, 2389???2401 (2010).

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114014/1

Member of these Collections

• iGEM Parts Registry