Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-18 05:04:22
aGFPnano_HA_aHelix_cgeA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2114017_sequence (Version 1) |
Description
N-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by an alpha helical linker.Notes
Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.Source
The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala et al. in 2010. [Ref]| Sequence Annotation | Location | Component / Role(s) |
| anti-GFP nanobody HA alpha helical linker cgeA | 1,354 355,381 382,414 415,816 | feature/cds CDS tag feature/tag feature/misc sequence_feature feature/cds CDS |