BBa_K2114020

BBa_K2114020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-18 08:59:53

CgeA_G4S_HA_GST



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2114020_sequence (Version 1)

    Description

    C-terminal fusion of glutathione S-transferase to spore crust gene cgeA by a flexible GGGGS linker.

    Notes

    Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

    Source

    The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

    Sequence Annotation Location Component / Role(s)
    cgeA
    GGGGS
    HA
    GST
    		1,399
    400,414
    415,441
    442,1143
    		feature/cds CDS
    feature/misc sequence_feature
    tag feature/tag
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114020/1
     

    Attachments

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