Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asli Azizoglu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-16 04:39:52
EsaR repressible promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K2116025_sequence (Version 1) |
Description
An esabox is an 18bp sequence to which the transcriptional regulator EsaR can bind. We placed one esabox right after the normally constitutive Anderson promoter [Bba_J23118] to create an EsaR repressible promoter. Transcription can be initiated by the specific AHL EsaR responds to [N-(3-oxo-hexanoyl)-L-homoserine lactone].Notes
The esabox has to be placed after the transcription start site to be able to act as a roadblock.Source
The Anderson promoter sequences were taken form the registry.Esabox sequence comes from the Pantoea stewartii ssp. stewartii.
| Sequence Annotation | Location | Component / Role(s) |
| Anderson Promoter, BBa_J23118 esabox | 1,35 41,58 | promoter feature/promoter non_covalent_binding_site feature/binding |