BBa_K212004

BBa_K212004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K212004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Elias Scheer

Date created: 2009-10-19 11:00:00

Date modified: 2015-05-08 01:11:29

secretion signal peptide + GFPmut3b

Details

• Types: DnaRegion
• Roles: Signalling; engineered_region
• Sequences: BBa_K212004_sequence (Version 1)

Description

This is a composite signalling part. The secretion signal peptide for beta-lactamase in S. epidermidis is ligated N-terminally to registry part BBa_E0040 (GFPmut3b). The signal peptide motif is used by the bacteria to target the protein for secretion through the Sec pathway. Any part may be put in place of GFP, but in its current state, the amount of secretion of any protein may be studied using confocal microscopy.

NOTE: This part only works for S. epidermidis ATCC 12228

Notes

Not Applicable.

Source

This part was synthesized based on a known sequence, which may be found here: .

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
secretion signal peptide	23,111	non_covalent_binding_site, feature/binding
GFPmut3b	118,836	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K212004/1

Member of these Collections

• iGEM Parts Registry