Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jinny van Doorn
Date created: 2016-10-19 11:00:00
Date modified: 2016-10-20 10:40:37
SPP1_GP35_Q38143
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2121003_sequence (Version 1) |
Description
Coding sequence for beta recombinase protein homolog.For use in recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (ie S. meliloti and CIAT 899). Identified with BLASTp from homology to the SPP1 35 (Q38143) B. subtillus phage beta-like recombination protein. Derived from phage that selectively infects R.
Within Yale iGEM 2016, known as gBlock 14, amplified for cloning with primers priG 459, 460.
Uniprot SPP1_GP35_Q38143 . E-value 4.90E-12
Notes
Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti.Source
Sequence ID: X97918.2, SPP1_GP35_Q38143| Sequence Annotation | Location | Component / Role(s) |
| cds SPP1 page recombination protein | 1,864 | feature/cds CDS |