Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jinny van Doorn
Date created: 2016-10-19 11:00:00
Date modified: 2016-10-20 10:47:07
Lambda_Beta_F7X3D1 (red_rhizo-MAGE)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2121004_sequence (Version 1) |
Description
Coding sequence for beta recombinase protein homolog.For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11)
Within Yale iGEM 2016, known as gBlock 15, amplified for cloning with primers priG 461, 462
Unitprot KB F7X3D1_SINMM, E value 1.50E-36
Notes
Elimination of any illegal restriction sites, and codon optimization for expression.Source
Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11). Gene: bet, Ordered Locus Names:SM11_chr1058| Sequence Annotation | Location | Component / Role(s) |
| cds recombination protein bet F7X3D1 | 1,951 | feature/cds CDS |