BBa_K2121008

BBa_K2121008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2121008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leslie Wang
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-29 08:58:52

Melibiose Inducible Promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K2121008_sequence (Version 1)

Description

Promoter regions in S. meliloti inducible by melibiose.

Notes

Utilize native suppression mechanism in S. meliloti.

Source

Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.

Sequence Annotation Location Component / Role(s)
trp terminator
alpha-galactoside utilization
alpha-galactosidase (melibiase) protein
LIC Upstream Site
citrine_ORF
LIC Downstream Site
T1 terminator
ORF 1
pVS1 staA
ORF 2
pVS1 repA
pBR322 origin
specR
spect
57,84
116,456
652,777
778,792
799,1518
1527,1541
1578,1646
2198,2884
3183,3812
4087,4681
4207,5314
6229,6848
7080,8012
7080,8079
stop_codon feature/stop
CDS feature/cds
CDS feature/cds
feature/misc sequence_feature
feature/cds CDS
sequence_feature feature/misc
feature/stop stop_codon
CDS feature/cds
feature/cds CDS
CDS feature/cds
feature/cds CDS
operator feature/operator
feature/cds CDS
CDS feature/protein
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2121008/1