BBa_K2121009

BBa_K2121009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2121009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leslie Wang
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-21 12:57:04

Rhamnose_Lambda_Beta_A6UAS9



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2121009_sequence (Version 1)

Description

Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).

Notes

Expression fusion with native S. meliloti protein.

Source

Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2121009/1