Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K2121009_sequence (Version 1)
|
Description
Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).
Notes
Expression fusion with native S. meliloti protein.
Source
Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.