BBa_K2121013

BBa_K2121013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2121013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leslie Wang

Date created: 2016-10-20 11:00:00

Date modified: 2016-10-29 09:37:10

AStr_Citrine_T1

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K2121013_sequence (Version 1)

Description

Anderson promoter, citrine fluorescence reporter, and T1 terminator cassette for rhizobia promoter characterization with LIC sites flanking the protein CDS.

Notes

Strong RBS that elevates citrine expression level in rhizobia.

Source

Anderson promoter and T1 terminator from iGEM registry. Citrine sequence from Stephen Dellaporta Lab, Yale University.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
promoter pJ23100	1,35	promoter, feature/promoter
RBS	36,39	ribosome_entry_site, feature/rbs
Upstream LIC cloning site	40,54	feature/misc, sequence_feature
citrine ORF	470,1841	CDS, feature/cds
terT1_LIC-R	1880,1916	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2121013/1

Member of these Collections

• iGEM Parts Registry