Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sannia Farrukh
Date created: 2016-09-28 11:00:00
Date modified: 2016-10-18 01:26:30
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K2122000_sequence (Version 1) |
Description
This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.Notes
An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.Source
Coding Sequence from GenBank| Access | Instance | Definition |
| public public public public | BBa_I0500 BBa_B0034 BBa_K2122150 BBa_B0014 | pBad/araC BBa_B0034 BBa_K2122150 BBa_B0014 |