BBa_K2122200

BBa_K2122200 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2122200
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sannia Farrukh
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-19 12:17:55

His-tagged Shiga-Like Subunit B toxin



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K2122200_sequence (Version 1)

Description

This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.

Notes

Thecode for his-tagged protein was added to both ends of the Gb3 synthase code

Source

Coding Sequence from GenBank

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2122200/1