BBa_K2124002

BBa_K2124002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2124002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brian Dempsey
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 08:19:23

Venom Serine Protease from Cerastes Cerastes - Thrombin analog



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Description

This Serine protease is a component of Cerastes Cerastes (Desert Horned Viper) venom. It recognizes Thrombin cut sites in fibrinogen and upon cleavage converts the fibrinogen to fibrin monomers. The fibrin monomers can then link and begin clot formation in mammals. This part contains a medium constitutive promoter and a secretion tag to send it to the periplasmic space.

Notes

This protein is very small and stabilized by several disulfide bonds. The final construct will be secreted into the periplasmic space with DsbC, a protein that shuffles disfulfide bonds. DsbC will allow the Serine Protease to fold properly with correct disulfide bonding. This will give stable functional protein.

Source

This is from a genomic sequence.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2124002/1