Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathryn Flanagan
Date created: 2016-10-15 11:00:00
Date modified: 2016-10-16 11:49:25
Strong promoter/RBS for MAHS
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2128104_sequence (Version 1) |
Description
A strong promoter and strong ribosome binding site were placed upstream of the MAHS LEA protein to maximize its expression.Notes
We have attempted to maximize expression of MAHS.Source
The coding sequence of this composite part is a member of a class of intrinsically disordered proteins called the Late Embryonic Abundant (LEA) proteins that become ordered when desiccated, thereby stabilizing DNA and protein cell components. This tardigrade unique protein is found in the mitochondria of tardigrade cells. When cloned into humans, they confer protection against osmotic stress as shown in the paper "Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells" by Sae Tanaka et al. 2015 Feb 12.It was taken from the genome of Ramazzottius varieornatus and optimized by the IDT Codon Optimization Tool for E. Coli K12.
| Access | Instance | Definition |
| public public | BBa_K880005 BBa_K2128004 | BBa_K880005 BBa_K2128004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K880005 BBa_K2128004 | 1,55 62,772 | BBa_K880005 BBa_K2128004 |