BBa_K2128204

BBa_K2128204 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2128204

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alejandro Schmieder, Alejandro Schmieder, Whitney Wong, David Sanderson

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-19 08:50:21

HDLEA1 with strong promoter/RBS

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K2128204_sequence (Version 1)

Description

A strong constitutive promoter (J23100) and strong ribosome binding site (B0034) have been placed upstream of the HDLEA1 in order to maximize expression of the protein to provide the ability to beter survive desiccation circumstances.

Notes

We have attempted to maximize expression of HDLEA1 through use of a strong constitutive promoter (J23100) and strong ribosome binding site (B0034).

Source

The coding sequence for HDLEA1 was taken from the genome of Ramazzottius varieornatus and optimized by the IDT Codon Optimization Tool for E. Coli K12

Components

Access	Instance	Definition
public	BBa_K880005	BBa_K880005
public	BBa_K2128004	BBa_K2128004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K880005	1,55	BBa_K880005
BBa_K2128004	62,334	BBa_K2128004

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2128204/1

Member of these Collections

• iGEM Parts Registry