BBa_K2129002

BBa_K2129002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2129002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maxwell Saal
Date created: 2016-10-12 11:00:00
Date modified: 2016-11-03 06:06:12

Mutant EutS with Mid expression cassette



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K2129002_sequence (Version 1)

    Description

    EutS with residue G26 replaced with an Amber stop codon for incorporation of non-canonical amino acids with a custom tRNA synthetase.

    Notes

    G26 was selected based on predictive modelling of EutS superstructure to incorporate at a locus where a phenylalanine-4'-azobenzene non-canonical amino acid might be able to hinder superstructure formation when under cis or trans conditions.

    Source

    EutS derived from K2129001, mutated with site directed mutagenesis (QuikChange).

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2129002/1
     

    Attachments

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