BBa_K2130001

BBa_K2130001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2130001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2016-10-17 11:00:00
Date modified: 2016-10-19 04:28:11

Cas9 mutant 462



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2130001_sequence (Version 1)

    Description

    This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.

    Notes

    No

    Source

    Addgene, the plasmid is then randomly mutated with EZClone Random mutation kit

    Sequence Annotation Location Component / Role(s)
    N869K
    E904K
    		2607,2607
    2710,2710
    		feature/misc sequence_feature
    feature/misc sequence_feature

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2130001/1
     

    Attachments

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