Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 12:51:48
LbCpf1 gRNA expression cassette
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2130008_sequence (Version 1) |
Description
The gRNA is expressed by the hU6 promoter. To insert gRNA, digest with BsmBI.To ligate in gRNA:
Design Top strand of your spacer with 5' AGAT overhang, bottom strand with 5'AAAA overhang.
Example:
5' AGATNNNNNNNNNNNNNNNN 3'
3' NNNNNNNNNNNNNNNNAAAA 5'