BBa_K2130010

BBa_K2130010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2130010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-19 06:19:11

∆
REC1-3 SP-dCas9

Details

• Types: DnaRegion
• Roles: Coding; CDS

Description

dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner.
For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta.

http://2016.igem.org/wiki/images/d/df/T--NTU-Singapore--rec1.png

http://2016.igem.org/wiki/images/1/1e/T--NTU-Singapore--rec1del.png

http://2016.igem.org/wiki/images/b/bb/T--NTU-Singapore--rec1delresult.png

http://2016.igem.org/wiki/images/8/81/T--NTU-Singapore--rec1micro.jpg

Notes

created by Gibson Assemly

Source

Addgene

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2130010/1

Member of these Collections

• iGEM Parts Registry