BBa_K2130013

BBa_K2130013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2130013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2016-10-23 11:00:00

Date modified: 2016-10-24 11:31:10

SpCas9

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2130013_sequence (Version 1)

Description

This past is a human condon optimised SpCas9. SpCas9 recognises an 5'-NGG-3' PAM at the 3' end of the target sequence.

Example(PAM in brackets):
5'-NNNN...NNN(NGG)-3'

Notes

Nope

Source

Addgene

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
V5 epitope	1,45	sequence_feature, feature/misc
SV40 NLS	49,72	feature/misc, sequence_feature
NLS	4195,4227	feature/misc, sequence_feature
Step. pyogenes Cas9	73,4194	sequence_feature, feature/misc

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2130013/1

Member of these Collections

• iGEM Parts Registry