BBa_K2130014

BBa_K2130014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2130014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2016-10-23 11:00:00

Date modified: 2016-10-24 11:43:33

SaCas9

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2130014_sequence (Version 1)

Description

This past is a human condon optimised Staph. aureus Cas9. SaCas9 recognises an 5'-NGGRRT-3' PAM at the 3' end of the target sequence.
Example(PAM in brackets):
5'-NNNN...NNN(NGGRRT)-3'
Evaluation studies from our team have compared the editing efficiency of this part compared to other Cas9/Cpf1.

Notes

nope

Source

Addgene

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SV40 NLS	7,30	feature/misc, sequence_feature
NLS	3208,3255	feature/misc, sequence_feature

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2130014/1

Member of these Collections

• iGEM Parts Registry