BBa_K2132001

BBa_K2132001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2132001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shijie Gu
Date created: 2016-08-25 11:00:00
Date modified: 2016-08-25 11:53:39

CsgASpyCatcherHisTag



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K2132001_sequence (Version 1)

Description

This is the subunit of the biofilm of E. Coli Curli system linked to SpyCatcher and HisTag. The two added parts can be used for various functions with SpyTag and other His tag-binding materials like quantum dots.

Notes

This part already includes the signal peptide for the secretion of CsgA.
Also, remember, this part has two His tags.

Source

CsgA comes from the genomic sequence of the E. Coli. We obtained the sequence of CsgA from the Timothy K. Lu lab.

Sequence Annotation Location Component / Role(s)
csgA with signal peptide and HisTag at N-teminal
Linker
SpyCatcher
His tag
1,471
472,501
502,846
847,867
CDS feature/cds
feature/dna sequence_feature
feature/tag tag
feature/tag tag
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2132001/1