BBa_K2132005

BBa_K2132005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2132005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YIFAN CHEN
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 05:45:15

HydA with SpyTag, Histag and TEV site



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2132005_sequence (Version 1)

    Description

    This is a modified protein, we added Histag and TEV site to the N-terminal site of hydA, and SpyTag to the C-terminal site of hydA.

    Notes

    We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

    Source

    The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

    Sequence Annotation Location Component / Role(s)
    SpyTag
    6x Histag
    TEV site
    HydA
    		1816,1857
    7,24
    25,45
    46,1791
    		feature/tag tag
    feature/tag tag
    feature/misc sequence_feature
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2132005/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)