BBa_K2132007

BBa_K2132007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2132007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YIFAN CHEN
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 06:05:34

HydF with Histag and TEV site



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2132007_sequence (Version 1)

    Description

    This is a modified protein, we added Histag and TEV site to the N-terminal site of hydF.

    Notes

    We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

    Source

    The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

    Sequence Annotation Location Component / Role(s)
    6x Histag
    TEV site
    hydF
    		7,24
    25,45
    46,1281
    		feature/tag tag
    sequence_feature feature/misc
    feature/cds CDS

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K2132007/1
     

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