BBa_K2139013

BBa_K2139013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2139013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: UBC iGEM 2016
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 04:02:56

Ptac with g12



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K2139013_sequence (Version 1)

Description

Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and is codon optimized to contain a high GC content.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Notes

Paenibacillus strain ICGEB2008

Source

It is codon optimized for Caulobacter cresentus, exhibiting a high GC content.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2139013/1