Types | DnaRegion
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Roles | engineered_region
Composite
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Sequences | BBa_K2139013_sequence (Version 1)
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Description
Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and is codon optimized to contain a high GC content.
Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154
Notes
Paenibacillus strain ICGEB2008
Source
It is codon optimized for Caulobacter cresentus, exhibiting a high GC content.