BBa_K2139015

BBa_K2139015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2139015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: UBC iGEM 2016

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-30 04:04:02

Endo5a with terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K2139015_sequence (Version 1)

Description

E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli.

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Notes

Acidothermus cellulolyticus

Source

Codon optimized for E. coli.

Components

Access	Instance	Definition
public	BBa_K2139001	BBa_K2139001
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2139001	1,1194	BBa_K2139001
BBa_B0015	1203,1331	BBa_B0015

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2139015/1

Member of these Collections

• iGEM Parts Registry