Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Robert Ashley Naturani
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 04:36:23
StarScafold NonSequential 1
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K2140005_sequence (Version 1) |
Description
Sequential intein activation is not required for enzyme splicing, we have a set of four previously tested inteins we can use for enzyme co-localisation which have been shown to have a high splicing rate, yield and orthologous splicing reactions. This part contains the coding sequence for our Non-Sequential StarScaffold, which allows us to check structural changes that occur due to splicing reactions occurring in the same mixture but at alternate temperatures.Notes
The length of the linker and leucine zipper regions along with the order of the intein segments.Source
The Leucine Zipper, Linker and Cystine regions we're designed denovo from the literature, while The inteins are a combination of engineered and non-engineered genomic gene segments.1. Stevens, A. J., Brown, Z. Z., Shah, N. H., Sekar, G., Cowburn, D., & Muir, T. W. (2016). Design of a Split Intein with Exceptional Protein Splicing Activity. Journal of the American Chemical Society, 138(7), 2162-2165.
2. Carvajal-Vallejos, P., Palliss??, R., Mootz, H. D., & Schmidt, S. R. (2012). Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. Journal of Biological Chemistry, 287(34), 28686-28696.
3. Dassa, B., London, N., Stoddard, B. L., Schueler-Furman, O., & Pietrokovski, S. (2009). Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family. Nucleic acids research, gkp095.
| Sequence Annotation | Location | Component / Role(s) |
| ATG (reverse compliment) | 1306,1308 | start_codon feature/start |