BBa_K214001

BBa_K214001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K214001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: QGEM_09

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:29

Pconst-RBS-Lpp-OmpA-Linker-TEV-TEV-Linker-ANP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K214001_sequence (Version 1)

Description

ANP outer membrane expression construct, with twin TEV cut sites designed for release of adhesion. Over expression of sequence can be toxic in bacteria.

Notes

Several restriction sites between domains to allow for future ease of modification.

Source

Combination of biobrick sequences Pconst(BBa_J23119) RBS (BBa_B0034), Lpp (BBa_J36835), OmpA (BBa_J36837), Linker (BBa_K157013-sequence between restriction sites), TEV cut site (BBa_K128002), ANP (consensus mature sequence, Genbank)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Pconst (J23119)	1,35	engineered_region, feature/BioBrick
Mlu I	588,593	sequence_feature, feature/misc
Linker (K157013-modified)	594,638	engineered_region, feature/BioBrick
BamHI	639,644	sequence_feature, feature/misc
ANP (K214002)	645,731	feature/BioBrick, engineered_region
Dual TEV cut site (K128002)	546,587	feature/BioBrick, engineered_region
Linker (K157013-modified)	495,539	feature/BioBrick, engineered_region
Sal I	489,494	feature/misc, sequence_feature
OmpA (J36837)	147,488	engineered_region, feature/BioBrick
Lpp (J36835)	54,140	feature/BioBrick, engineered_region
Hind III	48,53	sequence_feature, feature/misc
RBS (B0034)	36,47	engineered_region, feature/BioBrick
Xho I	540,545	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K214001/1

Member of these Collections

• iGEM Parts Registry