Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun
Date created: 2016-10-03 11:00:00
Date modified: 2016-10-29 07:47:08
Clover - alpha synuclein (1-60) - mRuby2 complex
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2141001_sequence (Version 1) |
Description
Clover is a yellow-green fluorescent protein derived from avGFP with increased efficiency, approximately 1.5 to 3 times more efficient than classical GFP and YFP reporters. mRuby2 is also a fluorescent protein with a red emission colour. mRuby2 is 2.5 times brighter than another red fluorescent protein mCherry. Both Clover and mRuby2 increase the efficiency when used in a FRET (F??rster resonance energy transfer) system compared to classical fluorescent proteins such as GFP, YFP and RFP. Alpha-synuclein is a neural protein found in presynaptic terminals and responsible from dopamine tuning in the presynaptic terminals. Alpha-synuclein is also said to be involved with methamphetamine. Methamphetamine binds to alpha synuclein's N-terminal domain and causes a conformational change. This complex together will help to detect the presence of methamphetamine by using the conformational change in the alpha-synuclein protein and the FRET effect caused by Clover-mRuby2 fluorescent proteins.Notes
We used Clover and mRuby2 in order to increase the FRET effect and codon optimizations of all three sequences in order to produce the protein in E.coli with maximum efficiency.Source
Alpha-synuclein is encoded by SNCA gene in human. Clover and mRuby2 proteins are synthetic fluorescent proteins derived from classical fluorescent proteins.| Sequence Annotation | Location | Component / Role(s) |
| Clover GGS linker alpha synuclein (1-93) GGS linker mRuby2 BamHI resctriction site | 1,717 718,726 727,1005 1006,1014 1015,1725 1726,1731 | feature/cds CDS feature/misc sequence_feature CDS feature/cds sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc |