BBa_K2141002

BBa_K2141002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2141002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 01:08:46

ECFP-meth scFv-EYFP

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2141002_sequence (Version 1)

Description

Methamphetamine binding syntetic antibody (scFv part) conjugated with ECFP and EYFP from N- and C- terminal of the scFv, designed to detect methamphetamine by using a FRET (f??rster resonance energy transfer) system involves ECFP and EYFP fluorescent proteins.

Notes

We specially designed the construct with the antibody at the center that when methamphetamine binds to the antibody, ECFP and EYFP will create FRET effect.

Source

It is ordered from IDT sponsorship. ScFv comes from mus musculus anti-meth antibody, ECFP and EYFP are the engineered enhanced forms of YFP and CFP fluorescent proteins.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	1,39	feature/rbs, ribosome_entry_site
ATG	40,42	start_codon, feature/start
ECFP	43,756	feature/cds, CDS
antibody chain 1	757,1089	feature/cds, CDS
linker	1090,1107	sequence_feature, feature/misc
antibody chain 2	1108,1458	CDS, feature/cds
EYFP	1459,2166	CDS, feature/cds
stop codon	2167,2169	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2141002/1

Member of these Collections

• iGEM Parts Registry