BBa_K2143000

BBa_K2143000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2143000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bryan Pasqualucci

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-15 11:44:32

NahR_L154F constitutive transcription factor binds upstream of Psal

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K2143000_sequence (Version 1)

Description

Codes for a mutant version of NahR transcription factor that binds upstream of Psal promoter. Wild Type version requires salicylate to induce Psal. This allele can induce Psal in the absence of salicylate. This part is useful to create a positive feedback loop when place downstream of Psal.

Notes

This part contains two copies of NahR_L154F. We did this because because we are using it as a positive feedback loop, we wanted the concentration of this mutant to be greater than the concentration of the inducer required allele.

Source

NCBI Reference Sequence: NC_007926.1

Components

Access	Instance	Definition
public	BBa_K2143001	BBa_K2143001
public	BBa_B0034	BBa_B0034
public	BBa_K2143001	BBa_K2143001
public	BBa_B0034	BBa_B0034

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2143001	1,903	BBa_K2143001
BBa_B0034	912,923	BBa_B0034
BBa_K2143001	930,1832	BBa_K2143001
BBa_B0034	1841,1852	BBa_B0034

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2143000/1

Member of these Collections

• iGEM Parts Registry