BBa_K2144008

BBa_K2144008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2144008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Oskar ??hman

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 01:11:52

Coding sequence for Sortase A with His-tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2144008_sequence (Version 1)

Description

Sortase A is a bacterial enzyme with the ability to break and form new peptide bonds. The key feature of the enzyme is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed.

This Biobrick also contains GB1 and a His-tag to enable IMAC-purification.

Notes

-

Source

iGEM 2016 isolated this part from S.Aureus

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GB1	1,164	feature/misc, sequence_feature
Sortase A	165,609	CDS, feature/protein
His-tag	610,625	feature/tag, tag

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2144008/1

Member of these Collections

• iGEM Parts Registry