Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Melody Wu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 02:59:37
GFP/RFP reporter with spacer (500bp)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2145002_sequence (Version 1) |
Description
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part (500bp) and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.Notes
Constructed with Golden Gate assembly.Source
Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 CAP -35 -10 LacI start BBa_B0034 conserved BBa_E1010 mrfp1 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_J04450 TetR 1 BBa_R0040 -35 TetR 2 -10 BBa_B0034 conserved BBa_E0040 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_I13522 | 1,200 89,126 137,142 161,166 166,200 173,173 209,220 213,216 227,907 227,901 908,932 941,1020 952,995 1029,1069 1036,1055 1056,1069 1062,1062 1,1069 1078,1096 1078,1131 1097,1102 1103,1121 1120,1125 1140,1151 1144,1147 1158,1877 1886,1965 1897,1940 1974,2014 1981,2000 2001,2014 2007,2007 1078,2014 | engineered_region feature/BioBrick feature/binding non_covalent_binding_site feature/promoter promoter promoter feature/promoter non_covalent_binding_site feature/binding start_codon feature/start engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/cds CDS feature/barcode engineered_tag feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region non_covalent_binding_site feature/binding feature/BioBrick engineered_region feature/promoter promoter feature/binding non_covalent_binding_site promoter feature/promoter engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon engineered_region feature/BioBrick |