Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samuel Clamons, Katie Maruna, Laura Chen, Melody Wu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 06:31:29
GFP/RFP reporter (Forward/Forward) separated by three dCas clamp sites
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2145100_sequence (Version 1) |
Description
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).Notes
Constructed with Golden Gate assembly.Source
Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13522 spacer gRNA_268 gRNA_355 gRNA_525 BBa_J04450 | 45,981 986,1235 1025,1047 1081,1103 1148,1170 1240,2308 | engineered_region feature/BioBrick feature/misc sequence_feature non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/BioBrick engineered_region |