GG105

BBa_K2145100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2145100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samuel Clamons, Katie Maruna, Laura Chen, Melody Wu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 06:31:29

GFP/RFP reporter (Forward/Forward) separated by three dCas clamp sites



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K2145100_sequence (Version 1)

Description

This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

Notes

Constructed with Golden Gate assembly.

Source

Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.

Sequence Annotation Location Component / Role(s)
BBa_I13522
spacer
gRNA_268
gRNA_355
gRNA_525
BBa_J04450
45,981
986,1235
1025,1047
1081,1103
1148,1170
1240,2308
engineered_region feature/BioBrick
feature/misc sequence_feature
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/BioBrick engineered_region
igem#experience
Issues
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2145100/1