BBa_K2148000

BBa_K2148000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2148000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chern Juin Ong, Ciara McCarthy
Date created: 2016-08-11 11:00:00
Date modified: 2016-10-04 06:11:25

psaA1 promoter + 5



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K2148000_sequence (Version 1)

Description

Promoter and 5'UTR sequence for the psaA gene in Chlamydomonas reinhardtii chloroplast, flanked with the Phytobrick fusion sites for a promoter with 5'UTR part. Submitted in the universal acceptor vector (psB1C3), and can be extracted using Bsa1 to give the necessary Phytobrick fusion site overhangs for Golden Gate assembly to produce a transcriptional unit with other Phytobricks.

Notes

Added Phytobrick fusion sites to flank promoter 5'UTR sequence

Source

Chlamydomonas reinhardtii chloroplast genome

Sequence Annotation Location Component / Role(s)
Phytobrick promoter prefix fusion site
psaA1 promoter
Phytbrick 5'UTR to CDS fusion site
5'UTR
1,4
5,149
284,287
150,283
engineered_region feature/BioBrick
feature/promoter promoter
engineered_region feature/BioBrick
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2148000/1