Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chern Juin Ong
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 01:23:59
gRNA for AGG PAM
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K2148015_sequence (Version 1) |
Description
This contains an sgRNA which has 20 nucleotide complementary sequences with the Chlamydomonas reinhardtii chloroplast genome upstream of the cut-site, which is at the trnE2-psbH integenic region with psaA promoter. This can be used together with Cas9 to perform site-specific cutting. This part is coded as a Promoter/5UTR/NTAG/CDS/CTAG Phytobrick.Notes
The target sequence was generated by Alex Mayorov using Benchling software and the scaffold sequence was obtained from Addgene.Source
Chlamydomonas reinhardtii| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick fusion site Promoter TSS Additional GG for stability target RNA gRNA scaffold Phytobrick fusion site | 1,4 5,149 149,149 150,151 152,171 172,254 255,258 | feature/BioBrick engineered_region promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature engineered_region feature/BioBrick |