Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aline Larissa Gon??alves
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 06:51:59
HPPD from Pseudomonas putida
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K2149001_sequence (Version 1) |
Description
This part coding a 4-hydroxyphenylpyruvate dioxygenase (HPPD) taken from Pseudomonas putida. This gene is involved in the pathway production of tocotrienol (vitamin E) and it is an oxygenase that catalyzes the second reaction in the catabolism of tyrosine - the conversion of 4-hydroxyphenylpyruvate into homogentisate (HGA). The HGA produced by 4-hydroxyphenylpyruvate dioxygenase that is one of many steps in break l-tyrosine into acetoacetate and fumarate.HPPD is part of a class of oxygenase enzymes that usually utilize α-ketoglutarate and diatomic oxygen to oxygenate or oxidize a target molecule. It is part of the cycle to create energy in almost aerobic organisms, but in eukaryotes, HPPD has a very important function that regulates blood tyrosine levels. Mutations in the N-terminal region of this protein cause the disease known as hawkinsinuria and HPPD can also be linked to one of the oldest known inherited metabolic disorders known as alkaptonuria.
Plants utilize this enzyme to produce the cofactors plastoquinone and tocopherol which are essential for the plant to survive, this is why there are several HPPD inhibitor herbicides that block the activity of this enzyme and cause the plant's death.
Notes
The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap.Source
This part was taken from the genomic sequence from Pseudomonas putida (access number K00457) that is a gram-negative, rod-shaped, saprotrophic soil bacterium. This gene is involved in the carotenoids pathway and was courtesy as a part of an operon that produces tocotrienol by Dr. Albermann.| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_K2149001 | 27,39 40,1117 | feature/rbs ribosome_entry_site CDS feature/cds |