BBa_K2149016

BBa_K2149016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2149016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andr?? Tomas Vilela Hermann

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 08:07:14

luxE (Acyl-protein synthetase)

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K2149016_sequence (Version 1)

Description

LuxE is an acyl-protein synthetase found in bioluminescent bacteria. LuxE catalyses the formation of an acyl-protein thiolester from a fatty acid and a protein. This is the second step in the bioluminescent fatty acid reduction system, which converts tetradecanoic acid to the aldehyde substrate of the luciferase-catalysed bioluminescence reaction [PMID: 8941351]. This enzyme takes the fatty acid substrate and catalyzes a reaction, forming a acyl-protein thioester product.
Together with the LuxC gene, represents the main reaction of the fatty aldehyde synthesis, which can then be used to produce alkanes.

Notes

The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap. Its design takes into consideration a RBS and a coding sequence.

Source

This part was synthesized by IDT, and it comes from the genomic sequence of Photorhabdus luminescens, codon optimized for Escherichia coli. The sequence was taken from the accession number JQ901710, which was used it to write the article "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli".

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	28,39	engineered_region, feature/BioBrick
luxE	40,1152	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2149016/1

Member of these Collections

• iGEM Parts Registry