BBa_K2150015

BBa_K2150015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2150015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianyi Huang
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 09:21:17

pTet TetX-GFP(fusion protein)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K2150015_sequence (Version 1)

Description

This part is the fusion protein of tetX-GFP with pTet promoter. PTet promoter is constitutively on and can be repressed by TetR. This part can report the expression of TetX with or without tetracycline.

Notes

The fusion protein can accurately report the expression level of TetX with fluorescence intensity of GFP. PTet is used to test the expression of tetX at the presence of tetracycline conveniently.

Source

PTet,RBS,TetX,GFP,double terminator all come from the kit.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2150015/1