| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K2150018_sequence (Version 1)
|
Description
Two promoters are used, a constitutive promoter pTet and a T7RNAP specific promoter pT7. By testing the fluorescence intensity, we found that in the absence of T7 promoter, this part exhibits higher efficiency than single pTet.
Notes
By adding a T7 promoter, we hope to achieve positive feedback in the presence of T7RNAP. We used GFP to test whether this can work before experimenting with our target gene.
Source
pT7 PCR amplified from BBa_K525998,pTet from BBa_R0040,RBS from BBa_B0034,GFP from BBa_E0040,DT from BBa_B0015
