pT7

BBa_K2150031 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2150031

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jianyi Huang

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-12 09:06:12

T7 promoter without RBS

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K2150031_sequence (Version 1)

Description

This part is the T7 promoter without RBS. It has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase(T7RNAP). However, we observed leakage of it in the absence of T7RNAP.

Notes

We deleted the RBS in BBa_K525998, so that RBS with different efficiency can be used.

Source

PCR amplified from BBa_K525998

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2150031/1

Member of these Collections

• iGEM Parts Registry