Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dekang Li
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 07:05:13
human Epidermal Growth Factor（
hEGF）
with FLAG-tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2152001_sequence (Version 1) |
Description
Human EGF（epidermal growth factor）is a 6045-Da protein with 53 amino acid residues. There is a phoA signal peptide at the N-terminus and a FLAG tag at the C-terminus of hEGF protein. PhoA is a fragment of signal peptide that guides the protein to go across cytomembrane and do not decrease activity of the protein. FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK.It can stimulate cell growth, proliferation and differentiation by binding to its receptor EGFR both in vivo and vitro.
This part is in charge of IBD treatment in our project.
Notes
There is a phoA signal peptide at the N-terminus and a FLAG tag at the C-terminus of hEGF protein. PhoA is a signal peptide that can guide protein to secrete to periplasmic space in E.coli.We can detect the FLAG tag on the protein by western blotting.